From lifespan, BioSciences LAMP1 is the Rabbit polyclonal antibody. It is used to recognize the antigen of rat, mouse, and human. There are different applications, use for the validation of LAMP1 antibody
. A few of these applications are:
Several suppliers are offering lamp1 antibodies. In humans, it encodes the lysosomal associated membrane protein 1. CD107a, LAMPA, LGP120, lysosome, and me-associated membrane glycoprotein 1 and lysosomal associated membrane protein 1b are the other names used for the lamp1 antibody. The LAMP1 gene used to indicate cancer. Fly, porcine, mouse, rat orthologs, porcine, and canin also found.
It is used to report the immunohistochemistry, immunoblotting, immunocytochemistry, and flow cytometric analysis. Inflow cytometric based degranulation assay, it may also be used to indicate the surface staining.
Background of LAMP1
In many cellular functions like cancer metastasis, LAMP1 has its implications. In some types of cancer, it acts as a therapeutic agent. It also marks different cells like cytotoxic T cells and lysosomal storage disorders.
LAMP2, that is also called CD17b, has a role in tumor cell metastasis. LAMP2 functions in adhesion, maintenance, and protection of lysosome. To increase the adhesion human peripheral blood mononuclear cells (PBMC) to vascular endothelium, LAMP1 and LAMP2 have been used, so in the site of inflammation, as they involved in adhesion of PBMC. In lysosomal membranes, LAMP1 also exist.
In post mortem frontal cortex (area 8) of stages, V-VIC of Braak and Braak, and Alzheimer's disease (AD) stages I-IIA/B and, compared with age-matched controls Protein level and LAMP1 mRNA, recent research was taken to LAMP1 mRNA testing. In multinucleated giant cells and microglia are found in an AD case, where the burden of amyloid was fine and beta A-peptide immunization also exist.
For some LRAP or leucine-rich amelogenin peptide and amelogenin isoform, LAMP1 also acts as a good cell surface receptor. On cementoblasts and dental follicle cells, LAMP1 also use as the binding site for cell surface for amelogenin.
Specification of LAMP1
LAMP1 and LAMP2 constitute the major lysosomal membrane, together. The plasma membrane has 1 t0 2 % LAMP1.LAMP1 contains a single membrane-spanning segment, N-linked glycosylation with 18 sites, putative signal peptide, and a cytosolic tail. LAMP proteins are used to fuse lysosomes with phagosomes and are also involved in lysosome biogenesis. LAMP1 transferred from trans-Golgi network to endosomes and after transported to lysosomes. The transfer of the LAMP1 Plasma membrane depends upon the carboxyl-terminal tyrosine-based motif (YXXI) when cell activates. LAMP1 and LAMP2 may be involved in adhesion of PBMC to the site of inflammation.
Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium; therefore, they are possibly involved in the adhesion of PBMC to the site of inflammation. Increased LAMP1 immunoreactivity is observed in neurons and glial cells surrounding senile plaques in Alzheimer's disease (AD) cases, and is localized in medullary epithelial cells, single macrophages, and lymphocytes in acute thymic involution. LAMP1 is a good marker of mast cell activation.